C57BL/6 Mouse Tracheal Smooth Muscle Cells -1 vial

Species : C57BL/6 Mouse Description: Mouse Tracheal Smooth Muscle Cells from AcceGen are isolated from tissue of pathogen-free laboratory mice Mouse Tracheal Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 05 hour and incubated in AcceGen’s Cell Culture Medium generally for 3-7 days Cultures are then expanded Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials Each vial contains at least 1×10^6 cells per ml and is delivered frozenMouse Tracheal Smooth Muscle Cells are characterized by immunofluorescent cell staining with antibodies of α-smooth muscle actin and are negative for bacteria, yeast, fungi, and mycoplasmaCells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGenRepeated freezing and thawing of cells is not recommendedStandard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applicationsand directly supplied to your lab by Gentaur's warehouse.