Aged Rat Primary Aortic Endothelial Cells -1 vial

Species : Rat Description: Aged Rat Primary Aortic Endothelial Cells from AcceGen are isolated from aorta of 74 week old laboratory Sprague-Dawley rat Aged Rat Primary Aortic Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in AcceGen’s Culture Complete Growth Medium generally for 3-7 days Cultures are then expanded Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials Each vial contains at least 1×106 cells per ml and is delivered frozen The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods These cells are pre-coated with PECAM-1 (CD31) antibody, following the application of magnetic beads pre-coated with secondary antibodyand directly supplied to your lab by Gentaur's warehouse.